Cell Line Catalog

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The DGRC has sought to collect as many cell lines as possible representing D. melanogaster and closely related species. The collection currently includes 159 lines and will continue to grow. We have posted protocols that we think will be useful to people using our lines. In addition, users who are not experienced with Drosophila cell culture may find it useful to read one or these reviews. For information about any individual line, we refer users to a list of published papers and a set of notes on the properties of the line, both accessible from links on the webpage for that cell line. In addition, our expression tool provides searchable modENCODE global expression data for 25 of the lines.


  • Showing:
Cell Line Stock # Species Tissue Source Special Features
1182-4H 177 melanogaster embryo haploid
modENCODE line
CCa 175 melanogaster embryo
CME L1 156 melanogaster prothoracic leg disc leg imaginal disc
modENCODE line
CME L1A 158 melanogaster prothoracic leg disc
CME L2 157 melanogaster prothoracic leg disc
CME W1 180 melanogaster wing disc
CME W1 Cl.8+ 151 melanogaster wing disc modENCODE line
CME W1 Cl.8R 162 melanogaster wing disc
CME W2 155 melanogaster wing disc modENCODE line
D1 10 melanogaster embryo
DeltaWTNdeMYC 153 melanogaster embryo Myc-tagged Delta
DH-33 23 hydei embryo
DM 173 melanogaster embryo
DX 176 melanogaster embryo
E-Adh-n1 12 melanogaster embryo Adhn-1
E-CS 22 melanogaster embryo Canton S
E-E12[ts] mal[1] 14 melanogaster embryo y l(1)E5CD1ts
E-FM1 20 melanogaster embryo
E-mal[1] 24 melanogaster embryo mal1
E-mal[F3] 17 melanogaster embryo malF3
E-OR 21 melanogaster embryo Oregon R
E-r[1] 15 melanogaster embryo r1
E-r[36] 18 melanogaster embryo r36
EH34A3 7 melanogaster embryo shits
fGS/OSS 191 melanogaster ovary co-culture of ovarian stem cells and somatic sheath cells
G1 16 melanogaster embryo
G2 4 melanogaster embryo
GM2 39 melanogaster embryo modENCODE line
GM3 11 melanogaster embryo
Jup-GFP, DSas-4 line 127 215 melanogaster embryo GFP-labeled microtubules
acentriolar
Jup-GFP, DSas-4 line 131 216 melanogaster embryo GFP-labeled microtubules
acentriolar
Jup-GFP, DSas-4 line 69 211 melanogaster embryo GFP-labeled microtubules
acentriolar
Jup-GFP, DSas-4 line 70 212 melanogaster embryo GFP-labeled microtubules
acentriolar
Jup-GFP, DSas-4 line 84 213 melanogaster embryo GFP-labeled microtubules
acentriolar
Jup-GFP, DSas-4 line 96 214 melanogaster embryo GFP-labeled microtubules
acentriolar
Jupiter 178 melanogaster embryo GFP-labeled microtubules
Kc167 1 melanogaster embryo modENCODE line
Kc167-IPPI-66D 233 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-IPPI-66D-Mt-mCherry 244 melanogaster embryo Mt-mCherry targeted to phiC31 docking site
Kc167-Mt-mCherry-c1 246 melanogaster embryo
Kc167-Mt-mCherry-c2 247 melanogaster embryo
Kc167-Mt-mCherry-c3 248 melanogaster embryo
Kc167-PP-16F 219 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-PP-21B 220 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-PP-21D 221 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-PP-50Aa 222 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-PP-50Ab 223 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-PP-52E 224 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-PP-61C 225 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-PP-89B 226 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-PP-93E 227 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc167-PP-93E-Mt-mCherry 245 melanogaster embryo Mt-mCherry targeted to phiC31 docking site
Kc167-PP-99A 228 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Kc7E10 182 melanogaster embryo secretes high level of extracellular matrix
L127D6 163 melanogaster prothoracic leg disc
L57-3-11 2 melanogaster embryo EcR-deficient
mbn2 147 melanogaster larval circulatory system tumorous blood cells
modENCODE line
MCW12 160 melanogaster wing disc
MDR3 8 melanogaster embryo aprt-deficient
ML-DmBG1 49 melanogaster central nervous system
ML-DmBG1-c1 50 melanogaster central nervous system modENCODE line
ML-DmBG2 51 melanogaster central nervous system
ML-DmBG2-c1 52 melanogaster central nervous system
ML-DmBG2-c10 62 melanogaster central nervous system
ML-DmBG2-c11 63 melanogaster central nervous system
ML-DmBG2-c12 64 melanogaster central nervous system
ML-DmBG2-c2 53 melanogaster central nervous system modENCODE line
ML-DmBG2-c3 54 melanogaster central nervous system
ML-DmBG2-c4 55 melanogaster central nervous system
ML-DmBG2-c5 56 melanogaster central nervous system
ML-DmBG2-c6 3 melanogaster central nervous system
ML-DmBG2-c8 60 melanogaster central nervous system
ML-DmBG3 66 melanogaster central nervous system
ML-DmBG3-c1 67 melanogaster central nervous system
ML-DmBG3-c2 68 melanogaster central nervous system modENCODE line
ML-DmBG3-c2-Mt-GFP-Act5C 174 melanogaster central nervous system GFP-Act5C fusion
ML-DmBG3-c3 69 melanogaster central nervous system
ML-DmBG8 77 melanogaster central nervous system
ML-DmD1-c3 81 melanogaster wing disc
ML-DmD1-c4 82 melanogaster wing disc
ML-DmD1-c5 122 melanogaster wing disc
ML-DmD1-c7 83 melanogaster wing disc
ML-DmD1-c8 84 melanogaster wing disc
ML-DmD11 94 melanogaster eye-antennal disc modENCODE line
ML-DmD11-c1 208 melanogaster eye-antennal disc
ML-DmD11-c2 209 melanogaster eye-antennal disc
ML-DmD11-c3 210 melanogaster eye-antennal disc
ML-DmD16-c1 96 melanogaster wing disc
ML-DmD16-c3 97 melanogaster wing disc modENCODE line
ML-DmD16-c4 98 melanogaster wing disc
ML-DmD16-c6 100 melanogaster wing disc
ML-DmD16-c7 101 melanogaster wing disc
ML-DmD16-c8 102 melanogaster wing disc
ML-DmD16-c9 103 melanogaster wing disc
ML-DmD17-c3 107 melanogaster haltere disc modENCODE line
ML-DmD20-c1 108 melanogaster antennal disc
ML-DmD20-c2 109 melanogaster antennal disc modENCODE line
ML-DmD20-c4 111 melanogaster antennal disc
ML-DmD20-c5 112 melanogaster antennal disc modENCODE line
ML-DmD21 86 melanogaster wing disc modENCODE line
ML-DmD23 121 melanogaster wing disc
ML-DmD23-c1 87 melanogaster wing disc
ML-DmD23-c2 116 melanogaster wing disc
ML-DmD23-c3 88 melanogaster wing disc
ML-DmD23-c4 89 melanogaster wing disc
ML-DmD23-c5 117 melanogaster wing disc
ML-DmD23-c6 118 melanogaster wing disc
ML-DmD25-c2 119 melanogaster wing disc
ML-DmD31 144 melanogaster wing disc
ML-DmD32 145 melanogaster wing disc modENCODE line
ML-DmD4-c1 126 melanogaster imaginal disc modENCODE line
ML-DmD8 92 melanogaster wing disc modENCODE line
ML-DmD9 85 melanogaster wing disc modENCODE line
ML81-18 25 melanogaster embryo
ML82-19a 27 simulans embryo
ML83-1b 31 teissieri embryo
ML83-26 38 melanogaster embryo
ML83-33 35 erecta embryo
ML83-61 46 melanogaster embryo mei9 mei41
ML83-62 41 melanogaster embryo
ML83-63 33 pseudoobscura embryo
ML83-68 34 auraria embryo
ML84-1 42 melanogaster embryo
ML84-27b 45 melanogaster embryo
ML84-29 44 melanogaster embryo
ML85-5 32 yakuba embryo
ML89-1 30 sechellia embryo
OSS 190 melanogaster ovary ovarian somatic sheath cells
PR8 204 melanogaster embryo
Pten 1 202 melanogaster embryo
Pten 1A 203 melanogaster embryo
Pten X 201 melanogaster embryo
Ras-attP-L1 249 melanogaster embryo phiC31 docking site
RMCE
Ras-attP-L2 250 melanogaster embryo phiC31 docking site
RMCE
Ras[V12]-H1 251 melanogaster embryo
Ras[V12]-H3 200 melanogaster embryo
Ras[V12]-H4 252 melanogaster embryo
Ras[V12]-H7 199 melanogaster embryo
Ras[V12]; wts[RNAi] 189 melanogaster embryo epithelial appearance
rumi[26] Ras[V12]-4 198 melanogaster embryo
S1 9 melanogaster embryo modENCODE line
S2-act-GFP-alphaTub84B 170 melanogaster embryo GFP-alphaTub84B fusion
S2-DGRC 6 melanogaster embryo
S2-DRSC 181 melanogaster embryo isolate of S2 used for RNAi in the DRSC
modENCODE line
S2-GFP-SKL 197 melanogaster embryo GFP targeted to peroxisomes
S2-Mt-Dl 152 melanogaster embryo Delta
S2-Mt-EB1-GFP 171 melanogaster embryo EB1-GFP fusion
S2-Mt-Fog-myc 218 melanogaster embryo secretes Fog-myc
S2-Mt-GFP 194 melanogaster embryo free GFP
S2-Mt-GFP-Act5C 169 melanogaster embryo GFP-Act5C fusion
S2-Mt-GFP-alphaTub84B 168 melanogaster embryo GFP-alphaTub84B fusion
S2-Mt-mCherry-alphaTub84B + Mt-GFP-Orbit 195 melanogaster embryo mCherry-tubulin fusion
GFP-Orbit fusion
S2-Mt-Msps-GFP 206 melanogaster embryo Msps-GFP fusion
S2-Mt-N 154 melanogaster embryo Notch
S2-Mt-Slit 192 melanogaster embryo Slit
S2-SQH-GFP 172 melanogaster embryo Sqh-GFP fusion
S2-SQH-GFP+Mt-mCherry-actin 193 melanogaster embryo Sqh-GFP fusion
mCherry-actin fusion
S2-Tub-wg 165 melanogaster embryo wingless
S2R+ 150 melanogaster embryo modENCODE line
S2R+-FUCCI 243 melanogaster embryo nuclear GFP
fluorescent markers for cell cycle
FUCCI
RFP
S2R+-NPT005 229 melanogaster embryo mCherry protein trap targeted to ER
RMCE
S2R+-NPT017 230 melanogaster embryo Fim-mCherry protein trap targeted to cleavage furrow
RMCE
S2R+-NPT050 231 melanogaster embryo mCherry protein trap targeted to peri-Golgi
RMCE
S2R+-NPT101 232 melanogaster embryo mCherry protein trap targeted to ER
RMCE
S2R+-SQH-GFP 196 melanogaster embryo Sqh-GFP fusion
S2R+-Ubi-pHMA 217 melanogaster embryo pH-sensitive GFP
engulfment assay
S3 5 melanogaster embryo modENCODE line
Sg4 179 melanogaster embryo modENCODE line
Sg4-PP-27F 238 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Sg4-PP-3A 237 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Sg4-PP-49B 239 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Sg4-PP-57B 241 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Sg4-PP-70F 242 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
Sg4-PP-84E 240 melanogaster embryo nuclear GFP
phiC31 docking site
RMCE
W20C6 159 melanogaster wing disc
WR Di-1 207 immigrans larva
WR Dv-1 40 virilis larva

Citing the DGRC

When publishing experiments using materials obtained from the DGRC please cite the Drosophila Genomics Resource Center, supported by NIH grant 2P40OD010949-10A1, in the acknowledgments. Your cooperation helps us when we need to renew our grant.