Cell Line Catalog

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video — Handling Cell Lines Video Tutorial

The DGRC has sought to collect as many cell lines as possible representing D. melanogaster and closely related species. The collection includes 257 lines and continues to grow. We have posted protocols that we think will be useful to people using our lines. In addition, users who are not experienced with Drosophila cell culture may find it useful to read one or these reviews. For information about any individual line, we refer users to a list of published papers and a set of notes on the properties of the line, both accessible from links on the webpage for that cell line.

Cell Line	Stock #	Species	Tissue Source	Special Features
1182-4H	177	melanogaster	embryo	haploid modENCODE line
24B>Ras attP GFP-L1	283	melanogaster	embryo	GFP RMCE-capable Expresses constitutively active Ras uncloned
24B>Ras attP-G1-CloneF3	325	melanogaster	embryo	RMCE GFP mesodermal
24B>Ras attP-G1-CloneG1	324	melanogaster	embryo	RMCE GFP mesodermal
24B>Ras attP-L5	284	melanogaster	embryo	RMCE-capable Expresses constitutively active Ras uncloned
24B>Ras attP-L5-CloneB8	323	melanogaster	embryo	RMCE mesodermal
Act5C-GS>Ras attP-GFP-LI-Clone2	330	melanogaster	embryo	RMCE GFP Neuronal
Act5C-GS>Ras attP-GFP-LI-Clone3	331	melanogaster	embryo	RMCE GFP hemocyte-like
Act5C-GS>Ras attP-LB-Clone6	329	melanogaster	embryo	RMCE Neuronal
btl>Ras attP-L3	332	melanogaster	embryo	Expresses constitutively active Ras DE-Cadherin positive tracheal epithelium uncloned
btl>Ras attP-L7	285	melanogaster	embryo	UAS-p35 RMCE-capable Expresses constitutively active Ras DE-Cadherin positive uncloned
btl>Ras attP-L8	286	melanogaster	embryo	UAS-p35 RMCE-capable Expresses constitutively active Ras DE-Cadherin positive uncloned
C6/36-HE8	336	Aedes albopictus	larva	RMCE mosquito
CCa	175	melanogaster	embryo
CME L1	156	melanogaster	prothoracic leg disc	leg imaginal disc modENCODE line
CME L1A	158	melanogaster	prothoracic leg disc
CME L2	157	melanogaster	prothoracic leg disc
CME W1	180	melanogaster	wing disc
CME W1 Cl.8+	151	melanogaster	wing disc	modENCODE line authenticated
CME W1 Cl.8R	162	melanogaster	wing disc
CME W2	155	melanogaster	wing disc	modENCODE line
CTCF19.7-1c	345	melanogaster	embryo
D	13	melanogaster	embryo	Replaced by 1182-4H
D1	10	melanogaster	embryo
delta l(3)mbt-OSC	289	melanogaster	ovary	CRISPR-Cas9 generated mutant derivative of OSC cultured cell line
DeltaWTNdeMYC	153	melanogaster	embryo	Myc-tagged Delta
DH-33	23	hydei	embryo
DM	173	melanogaster	embryo
DX	176	melanogaster	embryo
E-Adh-n1	12	melanogaster	embryo	Adh^n-1
E-CS	22	melanogaster	embryo	Canton S
E-FM1	20	melanogaster	embryo
E-mal[1]	24	melanogaster	embryo	mal¹
E-mal[F3]	17	melanogaster	embryo	mal^F3
E-OR	21	melanogaster	embryo	Oregon R
E-r[1]	15	melanogaster	embryo	r¹
E-r[36]	18	melanogaster	embryo	r³⁶
EH34A3	7	melanogaster	embryo	shi^ts
fGS/OSS	191	melanogaster	ovary	co-culture of ovarian stem cells and somatic sheath cells
G1	16	melanogaster	embryo
G2	4	melanogaster	embryo
GM2	39	melanogaster	embryo	modENCODE line
GM3	11	melanogaster	embryo
Jup-GFP, DSas-4 line 127	215	melanogaster	embryo	GFP-labeled microtubules acentriolar
Jup-GFP, DSas-4 line 131	216	melanogaster	embryo	GFP-labeled microtubules acentriolar
Jup-GFP, DSas-4 line 69	211	melanogaster	embryo	GFP-labeled microtubules acentriolar
Jup-GFP, DSas-4 line 70	212	melanogaster	embryo	GFP-labeled microtubules acentriolar
Jup-GFP, DSas-4 line 84	213	melanogaster	embryo	GFP-labeled microtubules acentriolar
Jup-GFP, DSas-4 line 96	214	melanogaster	embryo	GFP-labeled microtubules acentriolar
Jupiter	178	melanogaster	embryo	GFP-labeled microtubules
Kc167	1	melanogaster	embryo	modENCODE line authenticated
Kc167 (M3 FEx 2.5%)	313	melanogaster	embryo	adapted for serum free media
Kc167 (M3)	312	melanogaster	embryo	adapted for serum free media
Kc167 (M3BPYE FEx2.5%)	314	melanogaster	embryo	adapted for serum free media
Kc167-attP-25C6	317	melanogaster	embryo	RMCE
Kc167-attP-99F8	318	melanogaster	embryo	RMCE
Kc167-IPPI-66D	233	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-IPPI-66D-Mt-mCherry	244	melanogaster	embryo	Mt-mCherry targeted to phiC31 docking site
Kc167-Mt-mCherry-c1	246	melanogaster	embryo
Kc167-Mt-mCherry-c2	247	melanogaster	embryo
Kc167-Mt-mCherry-c3	248	melanogaster	embryo
Kc167-PP-16F	219	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-PP-21B	220	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-PP-21D	221	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-PP-50Aa	222	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-PP-50Ab	223	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-PP-52E	224	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-PP-61C	225	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-PP-89B	226	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-PP-93E	227	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc167-PP-93E-Mt-mCherry	245	melanogaster	embryo	Mt-mCherry targeted to phiC31 docking site
Kc167-PP-99A	228	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Kc7E10	182	melanogaster	embryo	secretes high level of extracellular matrix
L127D6	163	melanogaster	prothoracic leg disc
L57-3-11	2	melanogaster	embryo	EcR-deficient
mbn2	147	melanogaster	larval circulatory system	tumorous blood cells modENCODE line authenticated
MCW12	160	melanogaster	wing disc
MDR3	8	melanogaster	embryo	aprt-deficient
ML-DmBG1	49	melanogaster	central nervous system
ML-DmBG1-c1	50	melanogaster	central nervous system	modENCODE line
ML-DmBG2	51	melanogaster	central nervous system
ML-DmBG2-c1	52	melanogaster	central nervous system
ML-DmBG2-c10	62	melanogaster	central nervous system
ML-DmBG2-c11	63	melanogaster	central nervous system
ML-DmBG2-c12	64	melanogaster	central nervous system
ML-DmBG2-c2	53	melanogaster	central nervous system	modENCODE line
ML-DmBG2-c2-attP-25C6	319	melanogaster	central nervous system	RMCE
ML-DmBG2-c3	54	melanogaster	central nervous system
ML-DmBG2-c4	55	melanogaster	central nervous system
ML-DmBG2-c5	56	melanogaster	central nervous system
ML-DmBG2-c6	3	melanogaster	central nervous system
ML-DmBG2-c8	60	melanogaster	central nervous system
ML-DmBG3	66	melanogaster	central nervous system
ML-DmBG3-c1	67	melanogaster	central nervous system
ML-DmBG3-c2	68	melanogaster	central nervous system	modENCODE line authenticated
ML-DmBG3-c2-attP-25C6	320	melanogaster	central nervous system	RMCE
ML-DmBG3-c2-attP-99F8	321	melanogaster	central nervous system	RMCE
ML-DmBG3-c2-Mt-GFP-Act5C	174	melanogaster	central nervous system	GFP-Act5C fusion
ML-DmBG3-c3	69	melanogaster	central nervous system
ML-DmBG8	77	melanogaster	central nervous system
ML-DmD1-c3	81	melanogaster	wing disc
ML-DmD1-c4	82	melanogaster	wing disc
ML-DmD1-c5	122	melanogaster	wing disc
ML-DmD1-c7	83	melanogaster	wing disc
ML-DmD1-c8	84	melanogaster	wing disc
ML-DmD11	94	melanogaster	eye-antennal disc	modENCODE line
ML-DmD11-c1	208	melanogaster	eye-antennal disc
ML-DmD11-c2	209	melanogaster	eye-antennal disc
ML-DmD11-c3	210	melanogaster	eye-antennal disc
ML-DmD16-c1	96	melanogaster	wing disc
ML-DmD16-c3	97	melanogaster	wing disc	modENCODE line
ML-DmD16-c4	98	melanogaster	wing disc
ML-DmD16-c6	100	melanogaster	wing disc
ML-DmD16-c7	101	melanogaster	wing disc
ML-DmD16-c8	102	melanogaster	wing disc
ML-DmD16-c9	103	melanogaster	wing disc
ML-DmD17-c3	107	melanogaster	haltere disc	modENCODE line
ML-DmD20-c1	108	melanogaster	antennal disc
ML-DmD20-c2	109	melanogaster	antennal disc	modENCODE line
ML-DmD20-c4	111	melanogaster	antennal disc
ML-DmD20-c5	112	melanogaster	antennal disc	modENCODE line
ML-DmD21	86	melanogaster	wing disc	modENCODE line
ML-DmD23	121	melanogaster	wing disc
ML-DmD23-c1	87	melanogaster	wing disc
ML-DmD23-c2	116	melanogaster	wing disc
ML-DmD23-c3	88	melanogaster	wing disc
ML-DmD23-c4	89	melanogaster	wing disc
ML-DmD23-c5	117	melanogaster	wing disc
ML-DmD23-c6	118	melanogaster	wing disc
ML-DmD25-c2	119	melanogaster	wing disc
ML-DmD31	144	melanogaster	wing disc
ML-DmD32	145	melanogaster	wing disc	modENCODE line
ML-DmD4-c1	126	melanogaster	imaginal disc	modENCODE line
ML-DmD8	92	melanogaster	wing disc	modENCODE line
ML-DmD9	85	melanogaster	wing disc	modENCODE line
ML81-18	25	melanogaster	embryo
ML82-19a	27	simulans	embryo
ML83-1b	31	teissieri	embryo
ML83-26	38	melanogaster	embryo
ML83-33	35	erecta	embryo
ML83-61	46	melanogaster	embryo	mei9 mei41
ML83-62	41	melanogaster	embryo
ML83-63	33	pseudoobscura	embryo
ML83-68	34	auraria	embryo
ML84-1	42	melanogaster	embryo
ML84-27b	45	melanogaster	embryo
ML84-29	44	melanogaster	embryo
ML85-5	32	yakuba	embryo
ML89-1	30	sechellia	embryo
NAMRU2-CQ-01-1.7	335	Culex quinquefasciatus	ovary	RMCE mosquito
OSC	288	melanogaster	ovary	ovarian somatic sheath cells
OSC:eFH-piwi	322	melanogaster	ovary
OSS	190	melanogaster	ovary	ovarian somatic sheath cells authenticated
PR8	204	melanogaster	embryo
Psc3-1	340	melanogaster	embryo
Psc4-1	337	melanogaster	embryo	RMCE-capable Expresses constitutively active Ras
Pten 1	202	melanogaster	embryo
Pten 1A	203	melanogaster	embryo
Pten X	201	melanogaster	embryo
Ras-attP-L1	249	melanogaster	embryo	phiC31 docking site RMCE
Ras-attP-L2	250	melanogaster	embryo	phiC31 docking site RMCE
Ras[V12]-H1	251	melanogaster	embryo
Ras[V12]-H3	200	melanogaster	embryo
Ras[V12]-H4	252	melanogaster	embryo
Ras[V12]-H7	199	melanogaster	embryo
Ras[V12]; wts[RNAi]	189	melanogaster	embryo	epithelial appearance Expresses constitutively active Ras
repo>Ras bratdsRNA-L6	282	melanogaster	embryo	glial uncloned
repo>Ras bratdsRNA-L6-Clone2	326	melanogaster	embryo	glial
repo>Ras bratdsRNA-L6-Clone4	327	melanogaster	embryo	glial
repo>Ras bratdsRNA-L6-CloneF9	328	melanogaster	embryo	glial
rumi[26] Ras[V12]-4	198	melanogaster	embryo	Expresses constitutively active Ras
S1	9	melanogaster	embryo	modENCODE line
S12-26I3	342	melanogaster	embryo	RMCE-capable Expresses constitutively active Ras
S12-27IIb	339	melanogaster	embryo	RMCE-capable Expresses constitutively active Ras
S2-act-GFP-alphaTub84B	170	melanogaster	embryo	GFP-alphaTub84B fusion
S2-DGRC	6	melanogaster	embryo	authenticated
S2-DGRC-attP-25C6	316	melanogaster	embryo	RMCE
S2-DRSC	181	melanogaster	embryo	isolate of S2 used for RNAi in the DRSC modENCODE line authenticated
S2-dTat-KO	344	melanogaster	embryo
S2-GFP-SKL	197	melanogaster	embryo	GFP targeted to peroxisomes
S2-Mt-Dl	152	melanogaster	embryo	Delta
S2-Mt-EB1-GFP	171	melanogaster	embryo	EB1-GFP fusion
S2-Mt-Fog-myc	218	melanogaster	embryo	secretes Fog-myc
S2-Mt-GFP	194	melanogaster	embryo	free GFP
S2-Mt-GFP-Act5C	169	melanogaster	embryo	GFP-Act5C fusion
S2-Mt-GFP-alphaTub84B	168	melanogaster	embryo	GFP-alphaTub84B fusion
S2-Mt-mCherry-alphaTub84B + Mt-GFP-Orbit	195	melanogaster	embryo	mCherry-tubulin fusion GFP-Orbit fusion
S2-Mt-Msps-GFP	206	melanogaster	embryo	Msps-GFP fusion
S2-Mt-N	154	melanogaster	embryo	Notch
S2-Mt-Slit	192	melanogaster	embryo	Slit
S2-RFP-GFP-Atg8a	290	melanogaster	embryo	RFP-GFP-Atg8a
S2-SQH-GFP	172	melanogaster	embryo	Sqh-GFP fusion
S2-SQH-GFP+Mt-mCherry-actin	193	melanogaster	embryo	Sqh-GFP fusion mCherry-actin fusion
S2-Tub-wg	165	melanogaster	embryo	wingless
S2R+	150	melanogaster	embryo	modENCODE line authenticated
S2R+ (FEx 10%)	311	melanogaster	embryo	adapted for serum free media
S2R+ (FEx 2.5%)	310	melanogaster	embryo	adapted for serum free media
S2R+-Apc-KO-1G	271	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-Apc-KO-3E	272	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-Apc-KO-E5	304	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-Apc2-KO	267	melanogaster	embryo	CRISPR-Cas9 generated Apc2 (FBgn0026598) mutant derivative of S2R Drosophila cultured cell line
S2R+-arl8-GFP-4	291	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression lysosomes
S2R+-attP-99F8	315	melanogaster	embryo	RMCE
S2R+-CG8786-KO	269	melanogaster	embryo	CRISPR-Cas9 generated CG8786 (FBgn0036897) mutant derivative of S2R Drosophila cultured cell line
S2R+-Cnx99a-GFP-4	273	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression endoplasmic reticulum (ER)
S2R+-Fib-GFP-11	278	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression nucleolus
S2R+-Fib-GFP-12	279	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression nucleolus
S2R+-FUCCI	243	melanogaster	embryo	nuclear GFP fluorescent markers for cell cycle FUCCI RFP
S2R+-gig-KO	297	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-Gmap-GFP-4	276	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression Golgi (cis)
S2R+-Gmap-GFP-7	277	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression Golgi (cis)
S2R+-Golgin245-GFP-1	280	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression Golgi (trans)
S2R+-hairy-KO-BF3B	298	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-His2Av-mNeonGreen B11	295	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression
S2R+-IA2-KO	266	melanogaster	embryo	CRISPR-Cas9 generated IA-2 (FBgn0031294) mutant derivative of S2R Drosophila cultured cell line
S2R+-Lam-GFP-7	292	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression nuclear membrane (inner)
S2R+-Lamin-mNeonGreen D6	296	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression nuclear membrane (inner)
S2R+-Moe-KO-21B4	303	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-msl2-KO	341	melanogaster	embryo	mCherry protein trap targeted to ER RMCE
S2R+-MT::Cas9	268	melanogaster	embryo	mCherry protein trap targeted to ER S2R NPT005 stably transformed with a pMK33-based vector expressing Cas9
S2R+-NPT005	229	melanogaster	embryo	mCherry protein trap targeted to ER RMCE
S2R+-NPT005∆Dsh7	346	melanogaster	embryo	mCherry protein trap targeted to ER RMCE
S2R+-NPT005∆Dsh7∆Stbm8	348	melanogaster	embryo
S2R+-NPT005∆Dsh7∆Stbm8∆Fz16	347	melanogaster	embryo	mCherry protein trap targeted to ER RMCE
S2R+-NPT017	230	melanogaster	embryo	Fim-mCherry protein trap targeted to cleavage furrow RMCE
S2R+-NPT050	231	melanogaster	embryo	mCherry protein trap targeted to peri-Golgi RMCE
S2R+-NPT101	232	melanogaster	embryo	mCherry protein trap targeted to ER RMCE
S2R+-Pex19-KO-A1	306	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-polo-GFP-2	275	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression kinetochore
S2R+-Pten-KO-1A4	307	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-Rab11-GFP-14	274	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression endosomes
S2R+-Sec23-GFP-6	294	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression endoplasmic reticulum (ER)
S2R+-Slik-KO-22C2	308	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-spin-GFP-2	293	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression lysosomes
S2R+-SQH-GFP	196	melanogaster	embryo	Sqh-GFP fusion
S2R+-Tnks-KO	270	melanogaster	embryo	CRISPR-Cas9 generated Tnks (FBgn0027508) mutant derivative of S2R Drosophila cultured cell line
S2R+-Tnks-KO-10B	300	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-Tnks-KO-12A	299	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-Tnks-KO-3H	301	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-TSC1-KO-C5	305	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-Ubi-pHMA	217	melanogaster	embryo	pH-sensitive GFP engulfment assay
S2R+-Yki-KO-G7	309	melanogaster	embryo	CRISPR-Cas9 generated KO mutant
S2R+-ZnT63C-KO	265	melanogaster	embryo	CRISPR-Cas9 generated ZnT63C (FBgn0035432) mutant derivative of S2R Drosophila cultured cell line
S2R+Tom20-GFP-2	302	melanogaster	embryo	mCherry protein trap targeted to ER knock-in protein trap, constitutive Cas9 expression mitochondria
S3	5	melanogaster	embryo	modENCODE line
Sg4	179	melanogaster	embryo	modENCODE line
Sg4-PP-27F	238	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Sg4-PP-3A	237	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Sg4-PP-49B	239	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Sg4-PP-57B	241	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Sg4-PP-70F	242	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Sg4-PP-84E	240	melanogaster	embryo	nuclear GFP phiC31 docking site RMCE
Sua-5B-IE8	333	Anopheles coluzzii	larva	RMCE mosquito
Sua5B-IE8-Act::Cas9-2A-Neo	334	Anopheles coluzzii	larva	RMCE mosquito
W20C6	159	melanogaster	wing disc
WR Di-1	207	immigrans	larva
WR Dv-1	40	virilis	larva

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