The DGRC has received our first gRNA collection to our clone catalog from the Weizmann Knock Out project. As we accept further gRNA collections, they will be added to our catalog and listed below.
Weizmann gRNA Collection
This collection comes from the Weizmann Knock Out project (used to create the "WKO lines") as described in Meltzer et al., 2019. It contains ~850 plasmids with gRNA sequences cloned into pCFD4 or pCFD5. Selection of genes for this gRNA library were made based on expression data of developing MB-gamma neurons.
Phosphoinositide signaling gRNA Library
Phosphoinositide signaling gRNA library was created by the Padinjat Lab (Trivedi et al., 2020). The library contains two sgRNAs cloned into either pBFv6.2 and pBFv6.2B vectors targeting each of the 103 Phosphoinositide signaling genes. For every gene, one sgRNA targets the first coding exon (sg1, cloned into pBFv6.2) and another targets the last coding exon (sg2, cloned into pBFv6.2B). For every gene targeted in the collection, dual gRNA plasmids are also available, that carry both the sgRNA's (sg1 and sg2) in a single plasmid (pBFv6.2B). The gRNA library has been validated for the ability to generate deletions in S2R+ cells and flies.
Searching for gRNA Clones
You can enter the ID for your gRNA or associated gene in the product search field above. If you have multiple clone IDs, you may paste them into the batch query field below. If you are looking for clones in a particular collection, use the Collections menu to the left for any additional tips on finding clones in that collection.