Jordan Hall
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cell line photo

Thawing, Culturing, and Cryopreserving Drosophila Cell Lines

About the DGRC

The Drosophila Genomics Resource Center (DGRC) serves the Drosophila research community by: (a) collecting and distributing DNA clones and vectors; (b) collecting and distributing Drosophila cell lines; (c) developing and testing genomics technologies for use in Drosophila and assisting members of the research community in their use.

cDNAs and Vectors

The DGRC has assembled a large (over 1,000,000 and expanding) inventory of DNA clones through the generosity of individual community members, the BDGP, and the CuraGen Corporation. The collection includes transformation vectors, cDNA clones, and the CuraGen yeast two-hybrid collection. Clone collections and common vectors are listed in the catalog.

Cell Lines

The DGRC has sought to collect as many cell lines as possible representing D. melanogaster and closely related species. The collection currently includes 193 lines and will continue to grow.

Cell lines, with brief descriptions, are listed in the catalog.

Video Tutorials

The DGRC has begun a series of instructional video tutorials for working with DGRC products.

The DGRC is located within the Biology Department of Indiana University, and is supported by a grant from the National Institutes of Health (ORIP and NIGMS). The DGRC was created under the guidance of Dr Peter Cherbas and its work is directed by Dr Andrew Zelhof and an advisory board representing the Drosophila research community.


Sigma M3 Replacement

Due to the shortage in US supply of Shields and Sang M3 media (Sigma), we have tested several Drosophila cell lines using insect cell culture media from various suppliers. In general, when adapting to new media, we have observed that cells fare better when they are gradually adapted to their new media. Please get in touch with us for recommendations when dealing with specific cell lines.

Citing the DGRC

When publishing experiments using materials obtained from the DGRC please cite the Drosophila Genomics Resource Center, supported by NIH grant 2P40OD010949, in the acknowledgments. Some products have additional citations listed on the product page. Your cooperation helps us when we need to renew our grant.