DGRC Stock Number: 250


Donor: Sathiya Manivannan and Amanda Simcox

FlyBase Report: FBtc0000250

Cellosaurus Report: CVCL_5I25

Only available to not-for-profit labs

Use Provision Statement: By ordering this line you agree to the constraints on use outlined in Simcox-Cell-Lines-Use-Provision.docx


  • phiC31 docking site
  • RMCE


  • New Cell Lines
  • Dm Embryonic

Price: $340.00

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To cite Ras-attP-L2

When publishing experiments using Ras-attP-L2, please include the following details in your manuscript.

  • Materials and Methods
    Ras-attP-L2 (DGRC Stock 250 ; https://dgrc.bio.indiana.edu//stock/250 ; RRID:CVCL_5I25)
  • Acknowledgements
    • Drosophila Genomics Resource Center (NIH Grant 2P40OD010949)
  • References
    Manivannan et al., 2015, Genetics 201(4): 1319--1328 FBrf0230389 PMID:26500255
Recommended Medium
  • Schneider's + 10% FCS
  • M3+BPYE + 10% FCS
Species melanogaster
Primary Reference FBrf0230389
Lab Of Origin Amanda Simcox
Stage Source embryonic stage
Tissue Source embryo
Source Genotype: y[1] w[unspecified]; P{UAS-Ras85D.V12}, P{attP.w[+].attP}JB89B / P{Act5C-GAL4}17bFO1
Doubling Time
  • M3+BPYE + 10% FCS: 76 hours

All times are approximate (hrs, 25° unless otherwise noted).