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DGRC Stock Number: 181 RRID: RRID:CVCL_Z992 Synonyms: S2 , SL2 , Schneider's 2 , Schneider's line 2 Donor: N. Perrimon and B. Mathey-Prevot FlyBase Report: FBtc0000181 Cellosaurus Report: CVCL_Z992 Features:
Categories:
S2 Isolate BackroundAt the beginning of the modENCODE project, it was desirable to designate a single S2 isolate to be used throughout the project and by members of the community who wished to take advantage of modENCODE-produced data; the desired attributes of this isolate were that it be easy to culture and known to be sensitive to RNAi treatment. For this purpose, Norbert Perrimon donated to the DGRC the isolate of S2 cells that were used for RNAi-based screens at the DRSC; we named this isolate S2-DRSC. Perrimon had received S2 cells from several labs (including the Cherbas lab), and the relationship between S2-DRSC and S2-DGRC is unknown. Note: This line is named for the DRSC (Drosophila RNAi Screening Center). |
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To cite S2-DRSC
When publishing experiments using S2-DRSC, please include the following details in your manuscript.
- Materials and Methods
S2-DRSC (DGRC Stock 181 ; https://dgrc.bio.indiana.edu//stock/181 ; RRID:CVCL_Z992)
- Acknowledgements
- Drosophila Genomics Resource Center (NIH Grant 2P40OD010949)
- References
Schneider, 1972, J. Embryol. exp. Morphol. 27: 353--365 FBrf0024118 PMID:4625067
| modENCODE data | Sex | Copy Number Variation | sRNA |
|---|---|
| Recommended Medium |
|
| Species | D melanogaster |
| Parent Line | isolate of Schneider's line 2 |
| Primary Reference | FBrf0024118 |
| Lab Of Origin | Schneider |
| Stage Source |
late embryonic stage |
| Tissue Source |
embryo |
| Sex | male ( basis: expression levels of roX genes, msl-2 and traF; splicing patterns of tra and Sxl ) |
| Source |
Strain: Oregon R |
| Doubling Time |
All times are approximate (hrs, 25° unless otherwise noted). |
