modENCODE Sex Data

This table is taken from Hangnoh Lee, C. Joel McManus, Dong-Yeon Cho, Matthew Eaton, Fioranna Renda, Maria Patrizia Somma, Lucy Cherbas, Gemma May, Sara Powell, Dayu Zhang, Lijun Zhan, Alissa Resch, Justen Andrews, Susan Celniker, Peter Cherbas, Teresa Przytycka, Maurizio Gatti, Brian Oliver, Brenton Graveley, and David MacAlpine (2014): DNA copy number evolution in Drosophila, Genome Biology 2014, 15(8):R70.

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Cell Line X:A Y:A Gene Expression Levels (FPKM) Splicing events (PSI)
roX1 roX2 msl-2 traF tra
female
Sxl
female
Kc167 0.94 0.00 0.41 1.17 1.94 9.04 0.22 1.00
1182-4H 0.95 0.00 5.19 0.11 3.75 16.67 0.52 0.96
D8 1.01 0.00 0.77 0.22 3.64 7.99 0.35 0.87
D16-c3 0.87 0.00 2.88 0.00 5.94 16.75 0.38 0.71
D17-c3 0.84 0.00 0.23 0.13 6.79 6.93 0.74 0.56
D9 0.86 0.00 66.98 8.82 14.34 6.35 0.87 0.14
D4-c1 0.56 0.00 70.44 1.48 10.18 0.32 0.99 0.25
BG3-c2 0.56 0.63 0.16 29.65 19.79 0.54 1.00 0.00
Cl.8 0.50 0.34 212.07 38.37 20.95 0.54 1.00 0.00
D20-c5 0.53 0.00 19.06 4.94 11.13 0.00 1.00 0.00
L1 0.54 0.00 96.50 7.73 24.10 0.00 1.00 0.00
mbn2 0.61 0.00 156.02 11.54 22.64 0.00 1.00 0.00
S2-DRSC 0.55 0.01 8.17 51.43 16.08 0.00 1.00 0.00
S2R 0.68 0.00 0.00 29.60 13.27 0.48 1.00 0.00
S3 0.53 0.00 6.13 11.42 18.75 0.00 1.00 0.00
Sg4 0.54 0.00 106.46 18.82 29.44 0.00 1.00 0.00
W2 0.55 0.04 60.20 2.99 12.93 1.65 1.00 0.00
S1 0.52 0.24 198.00 1.05 18.26 0.00 1.00 NA

Table 1. Sex-determination of Drosophila cell lines based on DNA and RNA sequencing. X chromosome, or Y chromosome to Autosome ratio (DNA amount), expression levels of sex-specific genes, and quantified levels splicing events are summarized. Red and Blue shades indicate male or female characteristics, respectively, that are determined based on RNA-Seq analyses of 100 different fly lines (whole animals, p < 0.05, one sampled t-test). Expression levels are FPKM values. Please note that we used not the whole Y chromosome, but a 5 kb region with no obvious repeats (chrYHet:140,000-145,000) to avoid the mappability issue. PSI : Proportion Spliced In. D20-c5 cell line : 0.53 for X:A, 0 for Y:A ratios (no RNA-Seq result). The table is sorted based on the splicing event.


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