DGRC Stock Number: 273


Synonyms: Cnx99a-GFP-4/Cas9

Donor: DRSC/Bellen/Perrimon

FlyBase Report: FBtc0000273

Cellosaurus Report: CVCL_XF62


  • mCherry protein trap targeted to ER
  • knock-in protein trap, constitutive Cas9 expression
  • endoplasmic reticulum (ER)


  • New Cell Lines
  • Dm Embryonic

Price: $318.00

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To cite S2R+-Cnx99a-GFP-4

When publishing experiments using S2R+-Cnx99a-GFP-4, please include the following details in your manuscript.

  • Materials and Methods
    S2R+-Cnx99a-GFP-4 (DGRC Stock 273 ; https://dgrc.bio.indiana.edu//stock/273 ; RRID:CVCL_XF62)
  • Acknowledgements
    • Drosophila Genomics Resource Center (NIH Grant 2P40OD010949)
    • Drosophila RNAi Screening Center (NIH Grant 5R24OD019847)
  • References
    Drosophila Genomics Resource Center, 2018.7.9, Modified Drosophila S2R+ Cultured Cell Lines. FBrf0239411
Recommended Medium
  • M3+BPYE + 10% FCS
  • Schneider's + 10% FCS
Species melanogaster
Parent Line targeted mutant from S2R+-MT::Cas9
Primary Reference FBrf0229979
Lab Of Origin DRSC
Stage Source late embryonic stage
Tissue Source embryo

Doubling times have not been tested on any media.