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DGRC Stock Number: 150 RRID: RRID:CVCL_Z831 Synonyms: S2 , SL2 , Schneider's 2 , Schneider's line 2 Donor: D. Wheeler FlyBase Report: FBtc0000150 Cellosaurus Report: CVCL_Z831 Features:
Categories:
S2 Isolate BackroundThese cells were given by Imogen Schneider to Tadashi Miyake soon after the establishment of the original S2 line, and were stored frozen for most of the next 50 years. An aliquot of the cells was thawed by Yanagawa in the 1990s and found to express a receptor for Wingless, unlike other S2 cells that had been tested. He gave it the name S2R+, where R+ refers to the expression of Wingless receptor. Because of its history, we suspect that these cells are more similar to the original line established in the Schneider laboratory than any of the other S2 isolates in our collection. |
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To cite S2R+
When publishing experiments using S2R+, please include the following details in your manuscript.
- Materials and Methods
S2R+ (DGRC Stock 150 ; https://dgrc.bio.indiana.edu//stock/150 ; RRID:CVCL_Z831)
- Acknowledgements
- Drosophila Genomics Resource Center (NIH Grant 2P40OD010949)
- References
Schneider, 1972, J. Embryol. exp. Morphol. 27: 353--365 FBrf0024118 PMID:4625067
| modENCODE data | Sex | Copy Number Variation | sRNA |
|---|---|
| Recommended Medium |
|
| Species | D melanogaster |
| Parent Line | isolate of Schneider's line 2 |
| Primary Reference | FBrf0024118 |
| Lab Of Origin | Schneider |
| Stage Source |
late embryonic stage |
| Tissue Source |
embryo |
| Sex | male ( basis: expression levels of roX genes, msl-2 and traF; splicing patterns of tra and Sxl ) |
| Source |
Strain: Oregon R |
| Doubling Time |
All times are approximate (hrs, 25° unless otherwise noted). |
