S2R+-polo-GFP-2

DGRC Stock Number: 275

RRID: RRID:CVCL_UZ54

Synonyms: polo-GFP-2/Cas9

Donor: DRSC/Perrimon/Bellen

FlyBase Report: FBtc0000275

Cellosaurus Report: CVCL_UZ54

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Features:

  • mCherry protein trap targeted to ER
  • knock-in protein trap, constitutive Cas9 expression
  • kinetochore

Categories:

  • New Cell Lines
  • Dm Embryonic




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To cite S2R+-polo-GFP-2

When publishing experiments using S2R+-polo-GFP-2, please include the following details in your manuscript.

  • Materials and Methods
    S2R+-polo-GFP-2 (DGRC Stock 275 ; https://dgrc.bio.indiana.edu//stock/275 ; RRID:CVCL_UZ54)
  • Acknowledgements
    • Drosophila Genomics Resource Center (NIH Grant 2P40OD010949)
    • Drosophila RNAi Screening Center (NIH Grant 5R24OD019847)
  • References
    Drosophila Genomics Resource Center, 2018.7.9, Modified Drosophila S2R+ Cultured Cell Lines. FBrf0239411
Recommended Medium
  • M3+BPYE + 10% FCS
  • Schneider's + 10% FCS
Species melanogaster
Parent Line targeted mutant from S2R+-MT::Cas9
Primary Reference FBrf0229979
Lab Of Origin DRSC
Stage Source late embryonic stage
Tissue Source embryo

Doubling times have not been tested on any media.