DROSOPHILA modENCODE CONSORTIUM

The modENCODE consortium has agreed to use 4 cell lines for all of the projects that will be done with cell lines, and to handle these lines with standard protocols in order to maximize the uniformity of the cells used for experiments in the many labs that form the consortium. The protocols that are accessed from this page are intended to provide common procedures to maintain uniformity among the labs. Please stick as closely as you can to these procedures for modENCODE experiments, even though the cells may grow perfectly well with variant versions of the protocols. We will update these protocols from time to time, but we will keep the actual procedures for handling cells invariant for the duration of the modENCODE project.

• General Procedures
• General procedures
• Protocols for Individual Cell Lines
• Kc167 (Purchase Kc167)
• S2-DRSC (Purchase S2-DRSC)
• ML-DmBG3-c2 (Purchase ML-DmBG3-c2)
• CME W1 Cl.8+ (Purchase CME W1 Cl.8+)
• Reagents
• Medium for Drosophila tissue culture
• Fetal calf serum for Drosohila tissue culture
• Insulin for Drosophila cell culture
• Fly extract for Drosophila tissue culture