S2-DRSC

Source: DGRC, stock #181. When ordering, please also send an e-mail to the DGRC (dgrc@indiana.edu) stating that you are ordering these cells for modENCODE; this will ensure that you receive cells from lot 181A1, the lot that has been reserved for modENCODE. This lot is now available.

Note: These are the S2 cells that have been used extensively for RNAi screens at the DRSC. They were recently given to the DGRC for distribution.

Medium

Schneider's medium + 10% heat-inactivated FCS.

Note: S2 cells are not particularly sensitive to the lot of FCS. We recommend that everyone use the same lot of serum (see below) to maximize consistency within modENCODE, but this is less critical than for some of the other lines.

Thawing cells

As soon as possible after you receive them, thaw an ampoule of frozen cells in a 25 cm2 T-flask, precisely as described in the DGRC protocol [https://dgrc.bio.indiana.edu/include/file/ThawingCells.pdf].

S2-DRSC cells take very little time to begin to grow after thawing. Once they reach approximately 107 cells/ml (enough to cover the surface of the flask), they should be diluted 3-fold as follows: Using a Pasteur pipet, blow medium at the surface of the flask to dislodge the cells; S2-DRSC cells are surface-adherent, but can be easily dislodged by this procedure. Add 10 ml fresh medium to the 5 ml in the flask. Gently mix by pipetting up and down. Transfer 10 ml of the cell suspension to a 10 cm plate. The 5 ml remaining in the flask can be used as a back-up culture. It will take 1-2 weeks for the cells to reach their normal growth rate; they should not be used for experiments during that time.

General instructions for culture maintenance


Citing the DGRC

When publishing experiments using materials obtained from the DGRC please follow the citation guidelines on the material's stock page, including citing the Drosophila Genomics Resource Center, supported by NIH grant 2P40OD010949, in the acknowledgments. Your cooperation helps us when we need to renew our grant as well as the researchers that donate materials to the DGRC.