S2R+-Fib-GFP-12

DGRC Stock Number: 279

RRID: RRID:CVCL_XF64

Synonyms: Fib-GFP-12/Cas9

Donor: DRSC/Perrimon/Bellen

FlyBase Report: FBtc0000279

Cellosaurus Report: CVCL_XF64

Features:

  • mCherry protein trap targeted to ER
  • knock-in protein trap, constitutive Cas9 expression
  • nucleolus

Categories:

  • New Cell Lines
  • Dm Embryonic




Price: $340.00

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To cite S2R+-Fib-GFP-12

When publishing experiments using S2R+-Fib-GFP-12, please include the following details in your manuscript.

  • Materials and Methods
    S2R+-Fib-GFP-12 (DGRC Stock 279 ; https://dgrc.bio.indiana.edu//stock/279 ; RRID:CVCL_XF64)
  • Acknowledgements
    • Drosophila Genomics Resource Center (NIH Grant 2P40OD010949)
    • Drosophila RNAi Screening Center (NIH Grant 5R24OD019847)
  • References
    Drosophila Genomics Resource Center, 2018.7.9, Modified Drosophila S2R+ Cultured Cell Lines. FBrf0239411
Recommended Medium
  • M3+BPYE + 10% FCS
  • Schneider's + 10% FCS
Species melanogaster
Parent Line targeted mutant from S2R+-MT::Cas9
Primary Reference FBrf0229979
Lab Of Origin DRSC
Stage Source late embryonic stage
Tissue Source embryo

Doubling times have not been tested on any media.