DGRC Analysis Protocols
These protocols are for DGRC-1 Amplicon Transcriptome microarrays and the DGRC-2 Oligonucleotide microarrays. Protocols for other microarrays will be added as they become available. These are the recommended protocols for microarray use and analysis. These protocols will be improved and updated over
time. Only the newest protocols will be posted here.
This is a protocol detailing the entire production and Quality
Control procedures used for the DGRC-1 Amplicon Microarrays and DGRC-2
A recommended RNA isolation protocol which is used for
all DGRC RNA isolation.
3 Different labelling methods optimized by DGRC research Scientists; 1 Direct, 2 Indirect, 3 Dendrimer.
|Hybridization Trouble Shooting Guide
If you are having trouble with Hybridizations, this guide is meant to help
you get better results from our long experience doing hybridizations.
IMPORTANT NOTE: If you refer to protocols published on our website please cite the DGRC accordingly. Please use the date, author and title on the document.
Citing the DGRC
experiments using materials obtained from the DGRC please follow the
citation guidelines on the material's stock page, including citing the
Drosophila Genomics Resource Center, supported by NIH grant
2P40OD010949, in the acknowledgments. Your cooperation helps us when
we need to renew our grant as well as the researchers that donate
materials to the DGRC.