Cells were plated on concanavalin A, fixed, and stained with the monoclonal antibody 6-11B-1, followed by Cy3-labeled Fab fragments to visualize acetylated microtubules. Cells were then stained with Alexa488-conjugated monoclonal antibody DM1A to visualize total α-tubulin and Hoechst 33342 to stain DNA. S Rogers. (10-27-2023)