Zeocin as selection agent
Thaw cells and grow in T25 flask with 5 mL of M3 + BPYE (10% FCS) media supplemented with 0.1mg/ML Zeocin for 1 week. After 1 week, passage cells to fresh media with 0.01 mg/mL Zeocin.
(from Gorski Lab with modification on media). Gorski lab uses ESF921 media.