This cell line was developed by G. Amador at the DRSC (Harvard Medical School) in collaboration with B. Xia (Perrimon lab/HMS) and Y. Ahmed (Dartmouth). The starting cell line was S2R+-MT::Cas9. The cells were transiently transfected with a plasmid encoding an sgRNA targeting either Tnks and a homology donor that knocks in a selectable marker and disrupts the gene (two rounds of knockout and selection), followed by single-colony isolation and molecular characterization. Molecular evidence suggests that these isolates are knockouts of the targeted genes. The DRSC cell line IDs for these cell lines are Tnks-91. Cells are hygromycin-, puromycin- and neomycin-resistant but can be stably grown without selection or can be grown in 200 ng/uL hygromycin.