This cell line was developed by R. Viswanatha in the Perrimon lab (Harvard Medical School) to support CRISPR pooled-format screening in Drosophila cells, as described in Viswanatha et al. 2018. The starting cell line used to generate S2R+-MT::Cas9 was NPT005, which was created using MiMIC technology and is described in pubmed:22174071. N-terminally flag-tagged Cas9 driven by a metallothionein promoter was transfected into NPT005 and stable transfected cells were derived to generate S2R+ MT::Cas9. Attributes of this cell line include: S2R+ derivative; expresses mCherry::Clic fusion protein; attP sites present; pMT-Flag-Cas9. Growth conditions: maintain hygro selection (200 ng/ mL) to prevent loss of Cas9. DRSC/Perrimon lab uses the following: Schneider's media (Thermo 21720); 1X Penn/Strep (Thermo 15070063); 10% FBS (Thermo 16140071); 200 ng/uL (=246 uL/500 mL); Hygromycin B (Calbiochem 400051-1MU).