ML-BG3-c2 cells were stably transformed with a mixture of Mt-GFP-Act5C (a gift from Steve Rogers) and p8HCO; the transfected population was selected with methotrexate.
use of methotrexate
We routinely include methotrexate in the medium when growing these cells for an extended period of time. We do not include methotrexate during freezing, thawing, and shipping to avoid excessive stress to the cells.