Note
Per the Sekelsky Lab: pBUF has a 2-kb Ubiquitin promoter, which drives a moderate level of expression in all tissues; heat-shock causes about 3X increased transcription (Lee et al., MCB 8:4727, 1988). The ATG corresponding to the initial codon is followed by sequences encoding the FLAG epitope. Coding sequences can be cloned in-frame using the sites in the polylinker. Alternatively, one can use the NcoI site that overlaps the ATG, to produce a non-tagged polypeptide. Note: The anti-FLAG Ab's (M2 and M5) recognize endogenous Drosophila proteins with Mr ca. 50 kD. For more information please see this website: http://sekelsky.bio.unc.edu/Research/Vectors/Vectors.html