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Cell Maintenance
Maintain the ML-DmD16-c3 cell line between 3.0 x 10e6 cells/mL and 9.0 x 10e6 cells/mL. Cells can be removed from the substrate either with a cell scraper or by using a Pasteur pipette.
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Sex
ML-DmD16-c3 cells are female, by criteria of the expression levels of roX gene, msl-2 and traF, and the splicing patterns of tra and Sxl (Lee et al., 2014).
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tissue source
The line was derived from L3 wing discs (Ui et al., 1987). Transcriptome analysis suggests similarity to cells in the notal region, but not to adepithelial cells (Cherbas et al., 2011).