Tags: Cell
2007/02/19 05:38:49S2 growing in M3+BPYE + 10% FCS
Tags: Cell
2011/06/23 17:36:05Photos by Francesca Ceprani and Patrizia Somma, donated by Maurizio Gatti August, 2004. Interpretation by Maurizio Gatti: The cells of this line have eight metacetric/submetantric chromosomes; four are probably third chromosomes and four are second chromosomes. In addition, these S2 cells have two heteromorphic X chromosomes, a small acrocentric chromosome and one or two dot (fourth) chromosomes. In all the cells of this line, there is an exchange between the third chromosomes, resulting in two chromosomes with unequal arms: one with an abnormally long arm and the other with an abnormally short arm. One of the second chromosomes of this line has an abnormally short arm; the missing material is probably translocated on the small acrocentric chromosome, which may carry the centromere of either the X or the fourth chromosome. Moreover, in a fraction of the cells, one of the second chromosomes is split into two acrocentric chromosomes (arrows), possibly due to a translocation with breakpoints near the centromere of the second chromosome and close to the centromere of either the X or the fourth chromosome. Finally, the X chromosome with extra fluorescent material at its short arm might be the result of a translocation between the X and the fourth chromosome.
Tags: Karyotype
2010/03/29 12:00:51Range of karyotypes seen in S2. Photos by Francesca Ceprani and Patrizia Somma, donated by Maurizio Gatti August, 2004. Interpretation by Maurizio Gatti: The cells of this line have eight metacetric/submetantric chromosomes; four are probably third chromosomes and four are second chromosomes. In addition, these S2 cells have two heteromorphic X chromosomes, a small acrocentric chromosome and one or two dot (fourth) chromosomes. In all the cells of this line, there is an exchange between the third chromosomes, resulting in two chromosomes with unequal arms: one with an abnormally long arm and the other with an abnormally short arm. One of the second chromosomes of this line has an abnormally short arm; the missing material is probably translocated on the small acrocentric chromosome, which may carry the centromere of either the X or the fourth chromosome. Moreover, in a fraction of the cells, one of the second chromosomes is split into two acrocentric chromosomes (arrows), possibly due to a translocation with breakpoints near the centromere of the second chromosome and close to the centromere of either the X or the fourth chromosome. Finally, the X chromosome with extra fluorescent material at its short arm might be the result of a translocation between the X and the fourth chromosome.
Tags: Karyotype
2010/03/29 12:02:16