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History
made in the Simcox lab from embryos carrying Act5C-GAL4, UAS-Ras[V12], and UAS-wts[RNAi] transposons
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Note about Simcox lab cell lines
All of the cell lines in our collection that were generated in the Simcox lab were established in Schneider's medium + 10% FCS, and were maintained in the Simcox lab in that medium, with trypsinization at each transfer. We grow all of these lines in M3+BPYE + 10% FCS; M3+BPYE (which we make in house) is very similar to Schneider's medium, but in our experience is more reliable than commercial Schneider's medium. All of the lines grow well in M3+BPYE + 10% FCS. The lines vary considerably in their properties, including the necessity of using trypsin for dislodging the cells from the substrate. Information for individual lines is given on the cell line webpages where it is available, and will be updated as we acquire more experience with these lines.
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comments on growing this line
This line is slightly tricky to grow. Cells can be removed from the substrate with vigorous pipetting, or with trypsin treatment. The cells tend to form large, tight clumps when they are removed from the substrate by either technique; these are particularly obvious when viewing freshly thawed cells. The clumps are not a problem; cells gradually move from the clump to the substrate, where they form a beautiful epithelial-like single layer. It may take a couple of days for the outgrowth from the clump to be visible in freshly thawed cells. See photos (click on "photos" tab) to see a time course of this process.
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Sex
Ras[V12]; wts[RNAi] cells are male, by criteria of roX gene expression and Sxl splicing (Stoiber et al., 2016, G3, in press).
WRR-1 cells are male and contain a Y chromosome. (Fagegaltier et al., 2016)