Maintain Sg4 cell line between 2.0 x 10e6 cells/mL - 9.0 x 10e6 cells/mL.
Clone of S3. Higher expression of Abd-B in Sg4, and a much higher expression of defensins in S2.
Sg4 cells are male, by criteria of the expression levels of roX gene, msl-2 and traF, and the splicing patterns of tra and Sxl (Lee et al., 2014). The assignment was confirmed by roX gene expression and Sxl splicing (Stoiber et al., 2016, G3, in press).
Provenance of Sg4
Recent work based on whole-genome sequencing, TE profiling, and transcriptomics clustered Sg4 cells closest to S3 cells, raising the question of whether the Sg4 line is a lineage of S3, and not of S2, as previously documented (Hua et al, Genetics 2021).