ML-DmD1 was made in the Miyake laboratory from wing discs of late L3 (y v f mal); it is entirely unrelated to the embryonic line D1. ML0DmD1-c5 is a clone made from the line ML-DmD1.
comments on growing this line
cells adhere strongly to the plate surface. Trypsin seems to have no effect when washing off cells; must use cell scraper when transferring.