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History
S2 cells stably transformed to express wild-type Notch from a Cu-inducible metallothionein promoter
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Note
Transformed clone from S2, expresses Notch from a metallothionein promoter.
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Note
Clonal line, but expression of N is not uniform.
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use of methotrexate
We routinely include methotrexate in the medium when growing these cells for an extended period of time. We do not include methotrexate during freezing, thawing, and shipping to avoid excessive stress to the cells.