S2 cells stably transformed to express Myc-tagged Delta from a Cu-inducible metallothionein promoter
Note
S2 cell population transformed to express Myc-tagged Delta from a metallothionein promoter
use of methotrexate
We routinely include methotrexate in the medium when growing these cells for an extended period of time. We do not include methotrexate during freezing, thawing, and shipping to avoid excessive stress to the cells.