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History
S2 cells stably transformed to express wild-type Delta from a Cu-inducible metallothionein promoter
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Note
Transformed clone from S2, expresses Delta from a metallothionein promoter.
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use of methotrexate
We routinely include methotrexate in the medium when growing these cells for an extended period of time. We do not include methotrexate during freezing, thawing, and shipping to avoid excessive stress to the cells.