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Cell Maintenance
Maintain ML-DmBG3-c2 cell line between 2.5 x 10e6 cells/mL - 1.0 x 10e7 cells/mL. Do not let these cells over grow or you will not be able to recover them.
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History
ML-DmBG3 made from brain ventral ganglion of late L3 (y v f mal). Cloned from ML-DMBG3 in Miyake lab.
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modENCODE data
ML-DmBG3-c2 is one of the four lines chosen for extensive analysis by the modENCODE project. Data on transcripts, protein binding sites and replication patterns are posted on FlyBase; transcriptome data are also available through the expression tool on the DGRC site.
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Sex
ML-DmBG3-c2 cells are male, by criteria of the expression levels of roX gene, msl-2 and traF, and the splicing patterns of tra and Sxl (Lee et al., 2014). The assignment was confirmed by roX gene expression and Sxl splicing (Stoiber et al., 2016, G3, in press).