LOCUS pMCS_Donor 8524 bp ds-DNA circular 20-JUL-2015 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_feature 1129..1365 /label=DHFR /ApEinfo_fwdcolor=#400080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 12 offset 0 CDS complement(7539..8198) /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 850..1096 /label=hsp70 TATA /ApEinfo_fwdcolor=#808080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2223..2469 /label=hsp70 TATA(1) /ApEinfo_label=hsp70 TATA /ApEinfo_fwdcolor=#808080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4199..4445 /label=hsp 70 TATA /ApEinfo_fwdcolor=#808080 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 717..849 /label=UAS /ApEinfo_fwdcolor=#666666 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 2090..2222 /label=UAS(1) /ApEinfo_label=UAS /ApEinfo_fwdcolor=#666666 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 4066..4198 /label=UAS(2) /ApEinfo_label=UAS /ApEinfo_fwdcolor=#666666 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1371..2069 /label=SV40-polyA /ApEinfo_fwdcolor=#4c4c4c /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 3226..3924 /label=SV40-polyA(1) /ApEinfo_label=SV40-polyA /ApEinfo_fwdcolor=#4c4c4c /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 5610..6308 /label=SV40-polyA(2) /ApEinfo_label=SV40-polyA /ApEinfo_fwdcolor=#4c4c4c /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 659..698 /label=attB40-L /ApEinfo_fwdcolor=#000000 /ApEinfo_revcolor=#b3b3b3 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 12 offset 0 misc_feature complement(4015..4054) /label=attB40-R /ApEinfo_fwdcolor=#000000 /ApEinfo_revcolor=#191919 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 12 offset 0 misc_feature 2501..3217 /label=eGFP /ApEinfo_fwdcolor=#008000 /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 12 offset 0 misc_feature 4472..5599 /label=TK /ApEinfo_fwdcolor=#0080ff /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 12 offset 0 ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctACG 661 GGTGCCAGGG CGTGCCCTTG GGCTCCCCGG GCGCGTACCC GCggtggcgg ccgcgAGATC 721 CAAGCTTGCA TGCCTGCAGG TCGGAGTACT GTCCTCCGAG CGGAGTACTG TCCTCCGAGC 781 GGAGTACTGT CCTCCGAGCG GAGTACTGTC CTCCGAGCGG AGTACTGTCC TCCgagcgga 841 gactctagcG AGCGCCGGAG TATAAATAGA GGCGCTTCGT CTACGGAGCG ACAATTCAAT 901 TCAAACAAGC AAAGTGAACA CGTCGCTAAG CGAAAGCTAA GCAAATAAAC AAGCGCAGCT 961 GAACAAGCTA AACAATCTGC AGTAAAGTGC AAGTTAAAGT GAATCAATTA AAAGTAACCA 1021 GCAACCAAGT AAATCAACTG CAACTACTGA AATCTGCCAA GAAGTAATTA TTGAATACAA 1081 GAAGAGAACT CTGAATaggg aattgggAAT TGAGCTGatc gtctaGAAAT GGAACGAAGT 1141 AGCAATGAAG TCAGTAATCC AGTTGCTGGC AATTTTGTAT TCCCATCGAA CGCCACGTTT 1201 GGTATGGGAG ATCGCGTGCG CAAGAAATCC GGCGCCGCCT GGCAAGGTCA GATTGTCGGG 1261 TGGTACTGCA CAAATTTGAC CCCCGAAGGC TACGCCGTCG AGTCTGAGGC TCACCCAGGC 1321 TCAGTACAGA TTTATCCTGT TGCGGCGCTT GAACGCATCA ACTGActaga GGATCTTTGT 1381 GAAGGAACCT TACTTCTGTG GTGTGACATA ATTGGACAAA CTACCTACAG AGATTTAAAG 1441 CTCTAAGGTA AATATAAAAT TTTTAAGTGT ATAATGTGTT AAACTACTGA TTCTAATTGT 1501 TTGTGTATTT TAGATTCCAA CCTATGGAAC TGATGAATGG GAGCAGTGGT GGAATGCCTT 1561 TAATGAGGAA AACCTGTTTT GCTCAGAAGA AATGCCATCT AGTGATGATG AGGCTACTGC 1621 TGACTCTCAA CATTCTACTC CTCCAAAAAA GAAGAGAAAG GTAGAAGACC CCAAGGACTT 1681 TCCTTCAGAA TTGCTAAGTT TTTTGAGTCA TGCTGTGTTT AGTAATAGAA CTCTTGCTTG 1741 CTTTGCTATT TACACCACAA AGGAAAAAGC TGCACTGCTA TACAAGAAAA TTATGGAAAA 1801 ATATTTGATG TATAGTGCCT TGACTAGAGA TCATAATCAG CCATACCACA TTTGTAGAGG 1861 TTTTACTTGC TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG 1921 CAATTGTTGT TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 1981 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 2041 TCATCAATGT ATCTTATCAT GTCTGGATCg GATCTgccta gaactagtgG ATCCAAGCTT 2101 GCATGCCTGC AGGTCGGAGT ACTGTCCTCC GAGCGGAGTA CTGTCCTCCG AGCGGAGTAC 2161 TGTCCTCCGA GCGGAGTACT GTCCTCCGAG CGGAGTACTG TCCTCCgagc ggagactcta 2221 gcGAGCGCCG GAGTATAAAT AGAGGCGCTT CGTCTACGGA GCGACAATTC AATTCAAACA 2281 AGCAAAGTGA ACACGTCGCT AAGCGAAAGC TAAGCAAATA AACAAGCGCA GCTGAACAAG 2341 CTAAACAATC TGCAGTAAAG TGCAAGTTAA AGTGAATCAA TTAAAAGTAA CCAGCAACCA 2401 AGTAAATCAA CTGCAACTAC TGAAATCTGC CAAGAAGTAA TTATTGAATA CAAGAAGAGA 2461 ACTCTGAATa gggaattggg aAATTGAGCT GatcgTCTAG ATGGTGAGCA AGGGCGAGGA 2521 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA 2581 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT 2641 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA 2701 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 2761 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA 2821 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 2881 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA 2941 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA 3001 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC 3061 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC 3121 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC 3181 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA cTAGAGGATC TTTGTGAAGG 3241 AACCTTACTT CTGTGGTGTG ACATAATTGG ACAAACTACC TACAGAGATT TAAAGCTCTA 3301 AGGTAAATAT AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG 3361 TATTTTAGAT TCCAACCTAT GGAACTGATG AATGGGAGCA GTGGTGGAAT GCCTTTAATG 3421 AGGAAAACCT GTTTTGCTCA GAAGAAATGC CATCTAGTGA TGATGAGGCT ACTGCTGACT 3481 CTCAACATTC TACTCCTCCA AAAAAGAAGA GAAAGGTAGA AGACCCCAAG GACTTTCCTT 3541 CAGAATTGCT AAGTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT GCTTGCTTTG 3601 CTATTTACAC CACAAAGGAA AAAGCTGCAC TGCTATACAA GAAAATTATG GAAAAATATT 3661 TGATGTATAG TGCCTTGACT AGAGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA 3721 CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT 3781 GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA 3841 AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC 3901 AATGTATCTT ATCATGTCTG GATCggatcc cccgggctgc aggAATTCCT TAAGCCTAGG 3961 GGATCTTTCG AAAGATCTGG TACCGCTAGC CATATGGTCG ACCTCGAGAT CGATGTACGC 4021 GCCCGGGGAG CCCAAGGGCA CGCCCTGGCA CCCGCtcgac catgAGATCC AAGCTTGCAT 4081 GCCTGCAGGT CGGAGTACTG TCCTCCGAGC GGAGTACTGT CCTCCGAGCG GAGTACTGTC 4141 CTCCGAGCGG AGTACTGTCC TCCGAGCGGA GTACTGTCCT CCgagcggag actctagcGA 4201 GCGCCGGAGT ATAAATAGAG GCGCTTCGTC TACGGAGCGA CAATTCAATT CAAACAAGCA 4261 AAGTGAACAC GTCGCTAAGC GAAAGCTAAG CAAATAAACA AGCGCAGCTG AACAAGCTAA 4321 ACAATCTGCA GTAAAGTGCA AGTTAAAGTG AATCAATTAA AAGTAACCAG CAACCAAGTA 4381 AATCAACTGC AACTACTGAA ATCTGCCAAG AAGTAATTAT TGAATACAAG AAGAGAACTC 4441 TGAATaggga attgggAATT GAGCTGtCTA GATGGCTTCG TACCCCTGCC ATCAACACGC 4501 GTCTGCGTTC GACCAGGCTG CGCGTTCTCG CGGCCATAGC AACCGACGTA CGGCGTTGCG 4561 CCCTCGCCGG CAGCAAGAAG CCACGGAAGT CCGCCTGGAG CAGAAAATGC CCACGCTACT 4621 GCGGGTTTAT ATAGACGGTC CTCACGGGAT GGGGAAAACC ACCACCACGC AACTGCTGGT 4681 GGCCCTGGGT TCGCGCGACG ATATCGTCTA CGTACCCGAG CCGATGACTT ACTGGCAGGT 4741 GCTGGGGGCT TCCGAGACAA TCGCGAACAT CTACACCACA CAACACCGCC TCGACCAGGG 4801 TGAGATATCG GCCGGGGACG CGGCGGTGGT AATGACAAGC GCCCAGATAA CAATGGGCAT 4861 GCCTTATGCC GTGACCGACG CCGTTCTGGC TCCTCATATC GGGGGGGAGG CTGGGAGCTC 4921 ACATGCCCCG CCCCCGGCCC TCACCCTCAT CTTCGACCGC CATCCCATCG CCGCCCTCCT 4981 GTGCTACCCG GCCGCGCGAT ACCTTATGGG CAGCATGACC CCCCAGGCCG TGCTGGCGTT 5041 CGTGGCCCTC ATCCCGCCGA CCTTGCCCGG CACAAACATC GTGTTGGGGG CCCTTCCGGA 5101 GGACAGACAC ATCGACCGCC TGGCCAAACG CCAGCGCCCC GGCGAGCGGC TTGACCTGGC 5161 TATGCTGGCC GCGATTCGCC GCGTTTACGG GCTGCTTGCC AATACGGTGC GGTATCTGCA 5221 GGGCGGCGGG TCGTGGCGGG AGGATTGGGG ACAGCTTTCG GGGACGGCCG TGCCGCCCCA 5281 GGGTGCCGAG CCCCAGAGCA ACGCGGGCCC ACGACCCCAT ATCGGGGACA CGTTATTTAC 5341 CCTGTTTCGG GCCCCCGAGT TGCTGGCCCC CAACGGCGAC CTGTACAACG TGTTTGCCTG 5401 GGCCTTGGAC GTCTTGGCCA AACGCCTCCG TCCCATGCAC GTCTTTATCC TGGATTACGA 5461 CCAATCGCCC GCCGGCTGCC GGGACGCCCT GCTGCAACTT ACCTCCGGGA TGGTCCAGAC 5521 CCACGTCACC ACCCCCGGCT CCATACCGAC GATCTGCGAC CTGGCGCGCA CGTTTGCCCG 5581 GGAGATGGGG GAGGCTAACT GAATcTAGAG GATCTTTGTG AAGGAACCTT ACTTCTGTGG 5641 TGTGACATAA TTGGACAAAC TACCTACAGA GATTTAAAGC TCTAAGGTAA ATATAAAATT 5701 TTTAAGTGTA TAATGTGTTA AACTACTGAT TCTAATTGTT TGTGTATTTT AGATTCCAAC 5761 CTATGGAACT GATGAATGGG AGCAGTGGTG GAATGCCTTT AATGAGGAAA ACCTGTTTTG 5821 CTCAGAAGAA ATGCCATCTA GTGATGATGA GGCTACTGCT GACTCTCAAC ATTCTACTCC 5881 TCCAAAAAAG AAGAGAAAGG TAGAAGACCC CAAGGACTTT CCTTCAGAAT TGCTAAGTTT 5941 TTTGAGTCAT GCTGTGTTTA GTAATAGAAC TCTTGCTTGC TTTGCTATTT ACACCACAAA 6001 GGAAAAAGCT GCACTGCTAT ACAAGAAAAT TATGGAAAAA TATTTGATGT ATAGTGCCTT 6061 GACTAGAGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC 6121 TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT 6181 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 6241 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 6301 TCTGGATCgG ATCTcatcgt acccagcttt tgttcccttt agtgagggtt aattgcgcgc 6361 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 6421 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 6481 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 6541 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 6601 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 6661 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 6721 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 6781 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 6841 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 6901 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 6961 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 7021 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 7081 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 7141 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 7201 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 7261 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 7321 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 7381 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 7441 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 7501 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 7561 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 7621 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 7681 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 7741 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 7801 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 7861 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 7921 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 7981 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 8041 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 8101 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 8161 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 8221 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 8281 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 8341 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 8401 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 8461 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 8521 ccac //