LOCUS pCMA-EcR-A 7517 bp DNA circular DEFINITION expression plasmid for EcR-A. KEYWORDS actin promoter; Cherbas; EcR; T7 promoter. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers misc_feature 6..6 /vntifkey="21" /label=translation\start\for\EcR-A promoter 6524..7147 /vntifkey="30" /label=CMV\Promoter promoter 7158..7176 /vntifkey="30" /label=T7\promoter misc_marker complement(5158..6015) /vntifkey="22" /label=Amp-R /note="ORF: Frame #2 Start: atg Stop: taa" 3'UTR 2874..3302 /vntifkey="50" /label=SV40\t\intron\&\3'UTR rep_origin complement(4397..4920) /vntifkey="33" /label=pUC\origin rep_origin 3961..4159 /vntifkey="33" /label=SV40\ori enhancer 3631..3950 /vntifkey="9" /label=Polyoma\enh+ori misc_feature 1200..1883 /vntifkey="21" /label=LBD polyA_signal 3303..3561 /vntifkey="25" /label=SV40\large\T\polyA BASE COUNT 1948 a 1968 c 1828 g 1773 t ORIGIN 1 gatccatgtt gacgacgagt ggacaacaac agtcaaagca aaagctgtcg accttaccta 61 gccatatttt gctacaacaa caattagctg cctctgcagg cccatcttcc tcagtatccc 121 tatccccatc ttcttctgcc gccctgaccc tgcatgtagc cagtgctaat ggcggtgcaa 181 gagaaacaac ttctgcagcc gcagtcaagg ataaactaag gccgacaccc acagccatta 241 aaattgagcc catgccggac gtcatttccg taggcacggt ggccggagga tctagcgtag 301 ctactgttgt tgctcccgca gcaaccacaa cttccaataa gccaaattct acagcggcgc 361 cctcgacgtc ggctgcagct gcaaatggtc atttggtgct tgtgccgaac aaaagaccgc 421 gacttgatgt taccgaggac tggatgtcca ctcctagtcc aggcagcgtg cccagctctg 481 cgccgccact ttcgccttcc cccggctcgc agaatcacag ctacaacatg tccaacggct 541 atgcatcccc catgtccgcc ggaagctatg atccttacag tcctaccggc aaaacaggtc 601 gcgatgatct ctcgccttcg agcagcttga acggatactc ggcgaacgaa agctgcgatg 661 cgaagaagag caagaaggga cctgcgccac gggtgcaaga ggagctgtgc ctggtttgcg 721 gcgacagggc ctccggctac cactacaacg ccctcacctg tgagggctgc aaggggttct 781 ttcgacgcag cgttacgaag agcgccgtct actgctgcaa gttcgggcgc gcctgcgaaa 841 tggacatgta catgaggcga aagtgtcagg agtgccgcct gaaaaagtgc ctggccgtgg 901 gtatgcggcc ggaatgcgtc gtcccggaga accaatgtgc gatgaagcgg cgcgaaaaga 961 aggcccagaa ggagaaggac aaaatgacca cttcgccgag ctctcagcat ggcggcaatg 1021 gcagcttggc ctctggtggc ggccaagact ttgttaagaa ggagattctt gaccttatga 1081 catgcgagcc gccccagcat gccactattc cgctactacc tgatgaaata ttggccaagt 1141 gtcaagcgcg caatatacct tccttaacgt acaatcagtt ggccgttata tacaagttaa 1201 tttggtacca ggatggctat gagcagccat ctgaagagga tctcaggcgt ataatgagtc 1261 aacccgatga gaacgagagc caaacggacg tcagctttcg gcatataacc gagataacca 1321 tactcacggt ccagttgatt gttgagtttg ctaaaggtct accagcgttt acaaagatac 1381 cccaggagga ccagatcacg ttactaaagg cctgctcgtc ggaggtgatg atgctgcgta 1441 tggcacgacg ctatgaccac agctcggact caatattctt cgcgaataat agatcatata 1501 cgcgggattc ttacaaaatg gccggaatgg ctgataacat tgaagacctg ctgcatttct 1561 gccgccaaat gttctcgatg aaggtggaca acgtcgaata cgcgcttctc actgccattg 1621 tgatcttctc ggaccggccg ggcctggaga aggcccaact agtcgaagcg atccagagct 1681 actacatcga cacgctacgc atttatatac tcaaccgcca ctgcggcgac tcaatgagcc 1741 tcgtcttcta cgcaaagctg ctctcgatcc tcaccgagct gcgtacgctg ggcaaccaga 1801 acgccgagat gtgtttctca ctaaagctca aaaaccgcaa actgcccaag ttcctcgagg 1861 agatctggga cgttcatgcc atcccgccat cggtccagtc gcaccttcag attacccagg 1921 aggagaacga gcgtctcgag cgggctgagc gtatgcgggc atcggttggg ggcgccatta 1981 ccgccggcat tgattgcgac tctgcctcca cttcggcggc ggcagccgcg gcccagcatc 2041 agcctcagcc tcagccccag ccccaaccct cctccctgac ccagaacgat tcccagcacc 2101 agacacagcc gcagctacaa cctcagctac cacctcagct gcaaggtcaa ctgcaacccc 2161 agctccaacc acagcttcag acgcaactcc agccacagat tcaaccacag ccacagctcc 2221 ttcccgtctc cgctcccgtg cccgcctccg taaccgcacc tggttccttg tccgcggtca 2281 gtacgagcag cgaatacatg ggcggaagtg cggccatagg acccatcacg ccggcaacca 2341 ccagcagtat cacggctgcc gttaccgcta gctccaccac atcagcggta ccgatgggca 2401 acggagttgg agtcggtgtt ggggtgggcg gcaacgtcag catgtatgcg aacgcccaga 2461 cggcgatggc cttgatgggt gtagccctgc attcgcacca agagcagctt atcgggggag 2521 tggcggttaa gtcggagcac tcgacgactg catagcaggc gcagagtcag ctccaccaac 2581 atcaccacca caacatcgac gtcctgctgg agtagaaagc gcagctgaac ccacacagac 2641 ataggggaaa tggggaagtt ctctccagag agttcgagcc gaactaaata gtaaaaagtg 2701 aataactaat ggacaagcgt aaaatgcagt tatttagtct taagcctgca aatattacct 2761 attattcata caaattaaca tataatacag cctattaaca attacgctaa agcttctcga 2821 gggggggccc ggtaccgatc ctgggcggcc gcccagctag ctaggtagct agaggatctt 2881 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta 2941 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat 3001 tgttgtggta ttttagattc caacctatgg aacttatgaa tgggagcagt ggtggaatgc 3061 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac 3121 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga 3181 ctttccttca gaattggtaa gttttttgag tcatgctgtg tttagtaata gaactcttgc 3241 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga 3301 aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag 3361 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 3421 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 3481 gcatcacaaa tttcacaaat aaagcatttt tatcactgca ttctagttgt ggtttgtcca 3541 aactcatcaa tgtatcttat catgtctgga tcagcttcag aagatgggcg agggcctcca 3601 acacagtaat tttcctcccg actcttaaaa tagaaaatgt caagtcagtt aaggaggaag 3661 tggactaact gacgcagctg gccgtgcgac atcctctttt aattagttgc taggcaacgc 3721 cctccagagg gcgtgtggtt ttgcaagagg aagcaaaagc ctctccaccc aggcctagaa 3781 tgtttccacc caatcattac tatgacaaca gctgtttttt ttagtattaa gcagaggccg 3841 gggacccctg ggcccgctta ctctggagaa aaagaagaga ggcattgtag aggcttccag 3901 aggcaacttg tcaaaacagg actgcttcta tttctgtcac actgtctggc cctgtcacaa 3961 ggtccagcac ctccataccc cctttaataa gcagtttggg aacgggtgcg ggtcttactc 4021 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 4081 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 4141 gaggaggctt ttttggaggc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 4201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 4261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 4321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 4381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 4441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 4501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 4561 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt 4621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 4681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 4741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 4801 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 4861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 4921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 4981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 5041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 5101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 5161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 5221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 5281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 5341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 5401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 5461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 5521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 5581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 5641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 5701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 5761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 5821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 5881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 5941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 6001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 6061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 6121 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 6181 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 6241 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 6301 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 6361 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 6421 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 6481 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcgcgttg acattgatta 6541 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 6601 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 6661 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 6721 cgtcaatggg tggactattt acggtaaact gcccacttgg cagtacatca agtgtatcat 6781 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 6841 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 6901 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 6961 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 7021 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 7081 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct 7141 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgt accagatatt 7201 tcagaattcg ggagtacact cttcatggcg atatacaaga cacacacaag cacgaacacc 7261 cagttgcgga ggaaattctc cgtaaatgaa aacccaatcg gcgaacaatt catacccata 7321 tatggtaaaa gttttgaacg cgacttgaga gcggagagca ttgcggctga taaggtttta 7381 gcgctaagcg ggctttaata aaacgggctg cgggaccagt tttcatatca ctaccgtttg 7441 agttcttgtg ctgtgtggat actcctcccg acacaaagcc gctccatcag ccagcagtcg 7501 tctaatccag agacccg //