LOCUS act-DHFR 3767 bp DNA circular DEFINITION LFC's plasmid containing the actin-DHFR (R67) frag in pUC8. KEYWORDS actin promoter; Cherbas; DHFR; LFC. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria FEATURES Location/Qualifiers promoter complement(3602..3602) /vntifkey="30" /label=P(BLA) /note=" bla gene promoter " CDS complement(2710..3567) /vntifkey="4" /label=AP(R) /note=" bla gene- Ap(r) determinant " promoter complement(1588..1588) /vntifkey="30" /label=P(LAC) /note=" lac promoter " rep_origin 1948..1948 /vntifkey="33" /label=ORI /note=" RNaseH cleavage point " misc_feature complement(1227..1527) /vntifkey="21" /label=actin\material promoter complement(1313..1313) /vntifkey="29" /label=actin\5C\promoter mRNA complement(761..1313) /vntifkey="54" /label=transcript misc_feature complement(1217..1226) /vntifkey="21" /label=linker misc_feature complement(848..1218) /vntifkey="21" /label=fragment\from\plasmid\R67 misc_feature complement(695..829) /vntifkey="21" /label=SV40\fragment /note="SV40 bases 2670-2534" CDS complement(946..1179) /vntifkey="4" /label=DHFR BASE COUNT 916 a 956 c 967 g 928 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttggctgc aggtcgacgc 421 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc 481 accgcccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac 541 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg 601 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt 661 gatgccggcc acgatgcgtc cggcgtagag gatcgatcca gacatgataa gatacattga 721 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg 781 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttc tgctttaata 841 agatcctctc agcgggcgcg aagccgtccg ctggagcgac aagttgggct gtagtcggtg 901 atgcccggtt tgaccgttga gcggcgcact tcaaccttga actcagttga tgcgttcaag 961 cgccgcaaca ggataaatct gtactgagcc tgggtgagcc tcagactcga cggcgtagcc 1021 ttcgggggtc aaatttgtgc agtaccaccc gacaatctga ccttgccagg cggcgccgga 1081 tttcttgcgc acgcgatctc ccataccaaa cgtggcgttc gatgggaata caaaattgcc 1141 agcaactgga ttactgactt cattgctact tcgttccatt tccgaccttt cttgtgaact 1201 tctaggtgtt ctatgtggat ccggggtctc tggattagac gactgctggc tgatggagcg 1261 gctttgtgtc gggaggagta tccacacagc acaagaactc aaacggtagt gatatgaaaa 1321 ctggtcccgc agcccgtttt attaaagccc gcttagcgct aaaaccttat cagccgcaat 1381 gctctccgct ctcaagtcgc gttcaaaact tttaccatat atgggtatga attgttcgcc 1441 gattgggttt tcatttacgg agaatttcct ccgcaactgg gtgttcgtgc ttgtgtgtgt 1501 cttgtatatc gccatgaaga gtgtactccc gaattcgtaa tcatggtcat agctgtttcc 1561 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 1621 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 1681 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 1741 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 1801 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 1861 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 1921 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 1981 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 2041 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 2101 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 2161 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 2221 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 2281 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 2341 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 2401 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 2461 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 2521 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 2581 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 2641 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 2701 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 2761 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 2821 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 2881 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 2941 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 3001 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 3061 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 3121 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 3181 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 3241 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 3301 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 3361 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 3421 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 3481 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 3541 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 3601 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 3661 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 3721 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc //